Improving the carboxyamidomethyl ester for subtilisin A-catalysed peptide synthesis.

نویسندگان

  • Roseri J A C de Beer
  • Timo Nuijens
  • Lotte Wiermans
  • Peter J L M Quaedflieg
  • Floris P J T Rutjes
چکیده

A series of novel glycine esters was evaluated for efficiency in subtilisin A-CLEA-catalysed peptide synthesis. The reactivity of the easily accessible carboxyamidomethyl (Cam) ester was further enhanced by elongating it with an amino acid residue, thereby creating more recognition space for subtilisin A.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Efficient Synthesis of Biodiesel From Waste Cooking Oil Catalysed by Al2O3 Impregnated with NaOH

Due to the high price of virgin vegetable oils and the drawbacks of the homogeneous catalytic transesterification, in this work an economically profitable alternative process was proposed for biodiesel synthesis in which transesterification of the low-cost waste cooking oil (WCO) with methanol in a heterogeneous system was done. Alumina impregnated with sodium hydroxide was utilized as a solid ...

متن کامل

Efficient Synthesis of Biodiesel From Waste Cooking Oil Catalysed by Al2O3 Impregnated with NaOH

Due to the high price of virgin vegetable oils and the drawbacks of the homogeneous catalytic transesterification, in this work an economically profitable alternative process was proposed for biodiesel synthesis in which transesterification of the low-cost waste cooking oil (WCO) with methanol in a heterogeneous system was done. Alumina impregnated with sodium hydroxide was utilized as a solid ...

متن کامل

A Search for Peptide Ligase: Cosolvent-Mediated Conversion of Proteases to Esterases for Irreversible Synthesis of Peptides'

Serine and cysteine proteases (trypsin, chymotrypsin, papain, subtilisin) in the presence of certain concentrations of water-miscible organic solvents express no amidase activities. The esterase activities, however, remain significant. These modified enzymes can be used as peptide ligases in a kinetically controlled approach (aminolysis) for the stepwise synthesis and fragment coupling of pepti...

متن کامل

Synthesis of neopentyl glycol and ethylene glycol esters by fatty acids in the presence of acidic ion exchange resin catalyst

A most effective and less energy demanding method of producing fatty esters, diol esters, by esterifying fatty acids, with neopentyl and ethylenglycol alcohols in the presence of an acidic ion exchange resin catalyst(polyestyrendivinylbenzensulfated) was investigated at elevated temperature. In this process an azeotroping agent, toluene, was used to facilitate continuous removal of water by dis...

متن کامل

Synthesis, Characterization, and Biological Activity Studies on Fanlizhicyclopeptide A

The synthesis of a proline-rich cyclic heptapeptide, fanlizhicyclopeptide A (8), previouslyisolated from the fruits of Annona squamosa (sugar-apples), is described via coupling oftetrapeptide l-prolyl-l-tyrosyl-l-leucyl-l-proline methyl ester with tripeptide Boc-glycyl-lvalyl-l-proline followed by cyclization of the linear fragment having seven amino acid units.Structure of the synthesized cycl...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Organic & biomolecular chemistry

دوره 10 33  شماره 

صفحات  -

تاریخ انتشار 2012